The Resource Human stem cell manual : a laboratory guide, edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz

Human stem cell manual : a laboratory guide, edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz

Label
Human stem cell manual : a laboratory guide
Title
Human stem cell manual
Title remainder
a laboratory guide
Statement of responsibility
edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz
Contributor
Subject
Genre
Language
eng
Summary
Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses
Cataloging source
OPELS
Dewey number
616/.02774
Illustrations
illustrations
Index
index present
LC call number
QH588.S83
LC item number
H86 2007
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Label
Human stem cell manual : a laboratory guide, edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz
Publication
Bibliography note
Includes bibliographical references and index
http://library.link/vocab/branchCode
  • net
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Basic methods in stem cell culture -- Characterization of stem cells -- Differentiation of human embryonic cells -- Genetic manipulation of stem cells -- Advanced methods -- Setting up a laboratory -- Stem cells and society
  • Foreword by James Battey -- Foreword by Jeannie Fontana -- Preface -- Part I: Basic methods: Maintenance of stem cell cultures -- 1. Human Embryonic Stem Cell Culture -- 2. Feeder layers and feeder-free culture -- 3. Mouse Embryonic Fibroblast Feeder Cells -- 4. Cryopreservation of hESC -- Part II: Basic methods: Characterization of stem cells -- 5. Classical Cytogenetics: Karyotyping -- 6. Spectral Karyotyping and Fluorescent in situ Hybridization -- 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns -- 8. FACS/Cell sorting -- 9. Fluorescence Immunocytochemical Analysis of Stem Cells -- 10. Characterization of Stem Cells Using RT-PCR -- 11. Gene Expression Profiling of Stem Cells by Microarray -- 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice -- 13. Generation of HESC-Derived Teratomas -- Part III: Differentiation of stem cells -- 14. Embryoid Body Formation and Neuroepithelia Differentiation -- 15. Motor Neuron and Dopamine Neuron Differentiation -- 16. Oligodendrocyte Differentiation from hESC -- 17. Cardiac -- 18. Hematopoietic -- Part IV: Genetic manipulation of stem cells -- 19. Genetic Manipulation of hESC: Lentivirus Vectors -- 20. Methods developed for mouse ES cells -- Part V: Advanced methods -- 21. Derivation of human ES cells -- 22. Neural Stem Cell Culture -- 23. Transplantation of stem cells -- 24. In vitro fertilization -- 25. Functional characterization-neural cells -- Part VI: Practical issues in establishing a human ES cell laboratory -- 26. Setting Up a Facility for hESC Research -- Part VII: Essays on stem cell biology -- 27. Intellectual Property: Owning the Stem Cell -- 28. Ethical Concerns for Stem Cell Research -- 29. Guidelines for hESC Research Oversight (ESCRO) Committees
Control code
ocn162130510
Dimensions
unknown
Extent
1 online resource (xxiii, 461 pages)
Form of item
online
Isbn
9780123704658
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color)
http://library.link/vocab/ext/overdrive/overdriveId
117075:117177
http://library.link/vocab/recordID
.b22796277
Specific material designation
remote
System control number
  • (OCoLC)162130510
  • pebcs0080549888

Library Locations

    • Deakin University Library - Geelong Waurn Ponds CampusBorrow it
      75 Pigdons Road, Waurn Ponds, Victoria, 3216, AU
      -38.195656 144.304955
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