The Resource Laboratory methods in enzymology : protein part C, Edited by Jon Lorsch

Laboratory methods in enzymology : protein part C, Edited by Jon Lorsch

Label
Laboratory methods in enzymology : protein part C
Title
Laboratory methods in enzymology
Title remainder
protein part C
Statement of responsibility
Edited by Jon Lorsch
Contributor
Editor
Subject
Language
eng
Member of
Cataloging source
NMQU
Dewey number
  • 572/.70721
  • 572.6
Illustrations
illustrations
Index
index present
Literary form
non fiction
Series statement
Methods in enzymology,
Series volume
v. 541
Label
Laboratory methods in enzymology : protein part C, Edited by Jon Lorsch
Publication
Copyright
Bibliography note
Includes bibliographical references and indexes
http://library.link/vocab/branchCode
  • zbnus
  • mel
Carrier category
volume
Carrier MARC source
rdacarrier
Content category
  • text
  • still image
Content type MARC source
  • rdacontent
  • rdacontent
Contents
Section 1: Protein protocols/protein precipiation: TCA precipitation -- Section II: Protein protocols/protein pull-down methods: Coimmunoprecipitation of proteins from yeast -- Coupling antibody to cyanogen bromide-activated sepharose -- Analysis of protein-protein interactions by coimmunoprecipitation --Section III: Protein protocols/protein purification: Use and application of hydrophobic interaction chromatography for protein purification -- Hydroxyapatite chromatography: purification strategies for recombinant proteins -- Salting out of proteins using ammonium sulfate precipitation -- Using ion exchange chromatography to purify a recombinantly expressed protein -- Gel filtration chromatography (size exclusion chromatography) of proteins -- Section IV: Protein protocols/purification of membrane proteins: Expression and purification of membrane proteins -- Explantory chapter: choosing the right detergent -- Section V: Protein protocols/SDS PAGE: One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE) -- Coomassie blue staining -- Silver staining of SDS-polyacrylamide gel -- Section VI: Protein protocols/Standard in vitro assays for protein-nucleic acid interactions: Standard in vitro assays for protein-nucleic acid interactions-gel shift assays for RNA and DNA binding -- Protein filter binding -- Section VII: Protein protocolstroubleshooting protein expression: Explanatory chapter: troubleshooting recombinant protein expression: general -- Explanatory chapter: troubleshooting protein expression: what to do when the protein is not soluble -- Section VIII: Protein protocols/Western blotting: Western blotting using chemiluminescent substrates
Control code
000052824332
Dimensions
24 cm
Extent
xvii, 272 pages
Isbn
9780124201194
Media category
unmediated
Media MARC source
rdamedia
Other physical details
illustrations
http://library.link/vocab/recordID
.b29961464
System control number
  • (OCoLC)868642459
  • ci29961464

Library Locations

    • Deakin University Library - Melbourne Burwood CampusBorrow it
      221 Burwood Highway, Burwood, Victoria, 3125, AU
      -37.846510 145.115099
    • Deakin University Library - Geelong Waurn Ponds CampusBorrow it
      75 Pigdons Road, Waurn Ponds, Victoria, 3216, AU
      -38.195656 144.304955
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