The Resource Proteomics in practice : a guide to successful experimental design, edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker

Proteomics in practice : a guide to successful experimental design, edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker

Label
Proteomics in practice : a guide to successful experimental design
Title
Proteomics in practice
Title remainder
a guide to successful experimental design
Statement of responsibility
edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Contributor
Subject
Language
eng
Cataloging source
StDuBDS
Dewey number
572.64
Illustrations
illustrations
Index
index present
Literary form
non fiction
Nature of contents
bibliography
Label
Proteomics in practice : a guide to successful experimental design, edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Publication
Note
Previous ed.: 2002
Bibliography note
Includes bibliographical references and index
http://library.link/vocab/branchCode
  • net
Contents
1. History -- 2. Critical points -- 3. Proteomics strategies -- 4. Concept of experimental planning -- Pt. I. Proteomics technology -- 1. Electrophoretic techniques -- 2. Liquid chromatography techniques -- 3. Mass spectrometry -- 4. Functional proteomics: studies of protein-protein interactions -- Pt. II. Practical manual of proteome analysis -- Equipment, consumables, reagents -- Step 1: sample preparation -- Step 2: fluorescence difference gel electrophoresis -- Step 3: isoelectric focusing -- Step 4: SDS polyacrylamide gel electrophoresis -- Step 5: scanning of gels containing pre-labeled proteins -- Step 6: staining of gels -- Step 7: image analysis and evaluation of DIGE gels -- Step 8: Spot excision -- Step 9: sample destaining -- Step 10: protein destaining -- Step 10: preotein digestion -- Step 11 Microscale desalting and concentrating of sample -- Step 12: chemical derivatization of the peptide digest -- Step 13: MS analysis -- Step 14: calibration of MALDI-ToF MS -- Step 15: preparing for a database search -- Pt. III. Trouble shooting -- 1. Two-dimensional electrophoresis -- 2. Mass spectrometry -- Legal statements
Control code
000043002653
Dimensions
25 cm
Edition
2nd ed
Extent
xx, 482 p.
Isbn
9783527319411
Lccn
01452583
Other physical details
ill. (some col.)
http://library.link/vocab/recordID
.b24013559
System control number
  • (OCoLC)195752789
  • wiley3527319417

Library Locations

    • Deakin University Library - Geelong Waurn Ponds CampusBorrow it
      75 Pigdons Road, Waurn Ponds, Victoria, 3216, AU
      -38.195656 144.304955
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